General Project Outcomes
- Two Patents were submitted, the first has issued and been licensed. There is a continuance for some claims from the first so additional patents are expected to issue.
- Several US companies now use versions of the technology.
- A small business has been started using the technology at the SIUC research park.
- US soybean growers will lose less to disease using crops derived with these tools. The currently loose $0.5 billion a year to soybean sudden death syndrome.
Lightfoot D.A., Meksem K., P.T. Gibson. 2001. Soybeen Sudden Death Syndrome Resistant soybeans, soybean cyst nematode resistant soybeans and methods of breeding and identifying resistant plants. US Patent # 6,300,541
Meksem K. and D.A. Lightfoot. 2004 (anticipated). Novel polynucleotides and polypeptides relating to loci underlying resistance to soybean cyst nematode and methods of use thereof. Patent pending. #11/009,154.
Specific Outcomes
New methods were developed for selection of resistance genes in soybean have been discovered and developed.
- New Markers for the most valuable resistance genes in soybean have been discovered and developed.
- The tools were commercialized with a start-up marker assisted selection company and made available to all breeders.
- Cultivars with superior disease resistance have been developed and released (Schmidt et al., 1999).
- Large companies like Pioneer/Dupont and Monsanto/Asgrow are using the technology in house for about 1 million selections a year.
- Small companies are using the services for the spin-off company, Genome and agricultural Biotechnology, Research, Dunn-Richmond Business Incubator Room 174, Carbondale IL 62903-0312
Publications
Njiti, V., T.W. Doubler, R.J. Suttner, L. Gray, P. Gibson and D. A. Lightfoot. 1998. Loci underlying Resistance to Fusarium solani and soybean sudden Death Syndrome (SDS) Mapped in Near Isogenic Lines. Crop Science 38(3):472-477.
Schmidt, M.E., R.J. Suttner, J Kleinn, P.T. Gibson, D.A. Lightfootand O.Myers Jr.
1999. Registration of LS-G996 soybean germplasm resistant to soybean Sudden Death Syndrome (SDS) and Soybean Cyst Nematode race 3. Crop Science 39(3):598.
Prabhu, R.R., Njiti, V., J. E. Johnson, Schmidt M.E. Klein R.J. and D.A. Lightfoot. 1999. Selecting soybean cultivars for dual reistance to cyst nematode Sudden Death Syndrome with two DNA markers. Crop Science 39(4):982-987.
Gray, L.E., Achenbach, L.A., Duff, R.J. and D.A. Lightfoot. 1999. Pathogenicity of Fusarium solani f. sp. Glycines isolates on soybean and green bean plants. J. Phytopathology. 147:281-284.
Meksem K., Doubler T.W., Chang S.J.C., Chancharoenchai K ., R. Suttner, P. Cregan, P. Rao Arelli, PT. Gibson and D.A. Lightfoot. 1999. Clustering among genes underlying QTL for field resistance to Sudden Death Syndrome and cyst nematode race 3. Theor. Appl. Genet. 99:1131-1142.
Luo, Y., O. Myers, D. A. Lightfoot, and M.E. Schmidt. 1999. Root Colonization of soybean Cultivars in the Field by Fusarium Solani F. sp. Glycines. Plant Disease 83:1155-1159.
Meksem K., Ruben E., Hyten D., Triwitayakorn K., Lightfoot DA. 2001 Conversion of AFLP bands to high-throughput DNA markers. Mol Gen Genomics 265:207-214.
Meksem K., Pantzapangopolous P., Hyten, D., Njiti VN, Rao-Arelli AP, and Lightfoot DA. 2001. Conservation of AFLP Bands to high-throughput DNA markers. Mol Gen Genomics 265:207-214.
Meksem K., Pantzapangopolous P., Hyten, D., Njiti VN, Rao-arelli AP, and Lightfoot. 2001. Inoculum rate influences selection for field resistance to Sudden Death Syndrome in the greenhouse. Crop Science 41(1):1726-1723.
Published In Electronic Database
Lightfoot D.A. 1999. The complete nucleotide sequence of the RAPD band OI03512 from Clycine max. genbank October 2000, AF292390.
Meksem,K., Ruben,E. Lightfoot D.A. 2000. 2000. Glycine max allele of the A2D8 marker derived from the AFLP band ECCG-MAAC417 50 kpb from the susceptibility allele for rhg4. Genbank October 2000, AF286701.
Meksem K., Ruben E., Hyten D., Triwitayakorn K., Lightfoot DA. 2001. Conversation of AFLP bands to high-throughput DNA markers.
Edited Publications
Njiti, V. Schmidt, C., Schmidt, M. and lightfoot, D.A. 1997. Mapping loci underlying yield in Illionois. Soybeen Genetics Newsletter 24:136-138.
Doubler, T.W., Suttner, R.J., Chang, S.JC., Gibson P. T. and Lightfoot, D.A. 1997. Qualitative inheritance of quantive loci. Soybean Genetics Newsletter 24:139-141.
Njiti, V. TOrto, GA., Gray L.E. and Lightfoot, D.A. 1997. An effective greenhouse assay for field resistance to SDS. Soybean Genetics Newsletter 242:133-135.
Bell-Johnson, B.B., G. Garvey, J. Johnson K. Meksem and Lightfoot, D.A. 1998. Methods for High-throughput marker assisted selection for soybean. Soybean Genetics Newsletter 25:115-118.
Njiti, V., J.E. Johnson, G.a. Torto, L. Gray, and D. A. Lightfoot. 1999: Field Resistance to Sudden Death Syndrome is effective at low Inoculm concentration in Green house Assays.Soybean genetics Newsletter 27:20-25.
Magazine and Newspaper articles
"Super resistant soybeans may fight SDS" Soybean Digest January 1997 p28.
"Stalking High yield bean genes" The Furrow Sept 1997 p 17
"Improving Resistance and yield by gene maping" 1998 ISPOB Reo
"Conquering the stealth soybean killer" Soybean Digest Jan 1998 p 14
"SIUC biotechnologist receives new bredding method at workshop" Country Column, February 1998
"SIUC biotechnologist receives $1.3 million NSF grant, Country Column Nov,1998 p14
"Creating Superior Maps" Soybean Digest, June 1999,p11.
"Soybean Gene Golfing: Best Shot at Identifying Genetic Markers. ISCB Report p4. 1999.
"SIUC Soybean Center Growing" Southern Illinoisian Aug 31, 1999.
"Illinois Future is full of beans" So. Illinoisian. Feb 17, 2000.
"A growth Industry" So. Illinoisian July 2000.
"Soybean Gene Golfing" 2000 ISPOB Report
"New Methods for Resistance gene Selection" 2000 ISPOB Report
"ISA honor Industry leaders" Illinois Soybean Farmer Newsletter Dec. 2000
"On the Ground Floor" So. Illinoisian Jan 2001.
"Genomics Research Taking New Approach" So. Illinoisian July 2001
"SIUC receives The SDS Resistance Patents" Country Column, Chancellors Newsletter, November 2001.
